Paulo Bispo,
PhD
Massachusetts Eye and Ear
Assistant Scientist
Harvard Medical School
Assistant Professor of Ophthalmology
paulo_bispo@meei.harvard.edu
For investigator inquiries only
About My Research
Center/Research Area Affiliation
Biography
Dr. Bispo is a microbiologist dedicated to developing better ways to diagnose, treat, and prevent infections. With training in innovation in clinical and molecular microbiology, his work is at the translational interface between laboratory research and clinical practice. His research focuses on the use of state-of-the-art molecular genetic and genomic approaches to develop new, rapid, sensitive, and comprehensive diagnostic tests. He is also using a combination of genomic and phenotypic approaches to study the epidemiology of antibiotic-resistant infections, biofilm-formation on artificial devices, and the development of novel approaches for prophylaxis and treatment of ocular and otolaryngology infections. He serves as an assistant director for the Harvard Ophthalmology Infectious Diseases Institute (IDI), and manager of the IDI’s Translational Research Initiative.
Education
2005: BS, Biomedical Sciences, University of Araraquara, Brazil
2009: MS, Infectious Diseases, Federal University of Sao Paulo, Brazil
2013: PhD, Infectious Diseases, Federal University of Sao Paulo, Brazil
Postgraduate Training
2013-2018: Research Fellowship, Ophthalmology, Massachusetts Eye and Ear
Academic Appointments
2018-present: Member of the Faculty of Ophthalmology, Harvard Medical School
Honors
2017: Student Travel Grant, 19th International Society for Contact Lens research Symposium
2013-2015: Postdoctoral Fellowship, CAPES Foundation, Ministry of Education, Brazil, Program Science without Borders
2011: Johnson & Johnson Award, Co-author, Meeting of the Pan-American Association of Ophthalmology
2010-2011: Fellowship Award, CAPES Foundation, Ministry of Education, Brazil
2009-2013: Doctoral Research Fellowship, National Council for Scientific and Technological Development, CNPQ, Ministry of Science Brazil
2008, 2011, 2012: ASM Student and Postdoctoral Fellow Travel Grant, 48th, 51st, and 52nd ICAAC Annual Meetings, American Society for Microbiology
2007-2009: Master’s Degree Fellowship, Sao Paulo Research Foundation – FAPESP, Bra
Publications powered by Harvard Catalyst Profiles
- Characterization of the resistome and predominant genetic lineages of Gram-positive bacteria causing keratitis. Antimicrob Agents Chemother. 2024 Mar 06; 68(3):e0124723.
- Staphylococcus aureus activates NRLP3-dependent IL-1ß secretion from human conjunctival goblet cells using a toxin and toll-like receptors 2 and 1. Front Cell Infect Microbiol. 2023; 13:1265471.
- Molecular Detection and Typing of Treponema pallidum in Non-Ocular Samples from Patients with Ocular Syphilis. Ocul Immunol Inflamm. 2023 Oct 05; 1-5.
- Microbiology of Eye Infections at the Massachusetts Eye and Ear: An 8-Year Retrospective Review Combined With Genomic Epidemiology. Am J Ophthalmol. 2023 Nov; 255:43-56.
- Design of medical tympanostomy conduits with selective fluid transport properties. Sci Transl Med. 2023 04 05; 15(690):eadd9779.
- Fungal keratitis caused by Coniochaeta mutabilis-A case report. J Mycol Med. 2023 May; 33(2):101384.
- Antimicrobial Activity of an Fmoc-Plantaricin 149 Derivative Peptide against Multidrug-Resistant Bacteria. Antibiotics (Basel). 2023 Feb 15; 12(2).
- An All-in-One Highly Multiplexed Diagnostic Assay for Rapid, Sensitive, and Comprehensive Detection of Intraocular Pathogens. Am J Ophthalmol. 2023 06; 250:82-94.
- Molecular characterization of fungal endophthalmitis and keratitis caused by yeasts. Med Mycol. 2022 Dec 29; 61(1).
- Improved Detection of Herpesviruses from Diluted Vitreous Specimens Using Hydrogel Particles. Diagnostics (Basel). 2022 Dec 01; 12(12).
- Comparative In Vitro Activity of New Lipoglycopeptides and Vancomycin Against Ocular Staphylococci and Their Toxicity on the Human Corneal Epithelium. Cornea. 2023 May 01; 42(5):615-623.
- Targeted Killing of Ocular Streptococcus pneumoniae by the Phage Endolysin MSlys. Ophthalmol Sci. 2022 Dec; 2(4):100193.
- Population structure of ocular Streptococcus pneumoniae is highly diverse and formed by lineages that escape current vaccines. Microb Genom. 2022 03; 8(3).
- A Systematic Review of Multi-decade Antibiotic Resistance Data for Ocular Bacterial Pathogens in the United States. Ophthalmol Ther. 2022 Apr; 11(2):503-520.
- Novel Molecular Barcoding for Rapid Pathogen Detection in Infectious Keratitis. Ophthalmol Sci. 2021 Dec; 1(4):100066.
- Diphtheroids as Corneal Pathogens in Chronic Ocular Surface Disease in Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis. Cornea. 2021 06 01; 40(6):774-779.
- Community-genotype methicillin-resistant Staphylococcus aureus skin and soft tissue infections in Latin America: a systematic review. Braz J Infect Dis. 2021 Jan-Feb; 25(1):101539.
- A Cluster of Corneal Donor Rim Cultures Positive for Achromobacter Species Associated With Contaminated Eye Solution. Cornea. 2021 Feb 01; 40(2):223-227.
- The Search for Antifungal Prophylaxis After Artificial Corneal Surgery-An In Vitro Study. Cornea. 2020 Dec; 39(12):1547-1555.
- Characterization and distribution of viridans group streptococci isolated from infectious endophthalmitis and keratitis. Arq Bras Oftalmol. 2020 Nov-Dec; 83(6):463-472.
- Coexistence of the Oxazolidinone Resistance-Associated Genes cfr and optrA in Enterococcus faecalis From a Healthy Piglet in Brazil. Front Public Health. 2020; 8:518.
- Application of Metagenomic Sequencing in the Diagnosis of Infectious Uveitis. Semin Ophthalmol. 2020 Aug 17; 35(5-6):276-279.
- Hospital-Associated Multidrug-Resistant MRSA Lineages Are Trophic to the Ocular Surface and Cause Severe Microbial Keratitis. Front Public Health. 2020; 8:204.
- Transferable Resistance Gene optrA in Enterococcus faecalis from Swine in Brazil. Antimicrob Agents Chemother. 2020 05 21; 64(6).
- Validation of a Comprehensive Clinical Algorithm for the Assessment and Treatment of Microbial Keratitis. Am J Ophthalmol. 2020 06; 214:97-109.
- The Best of All Worlds: Streptococcus pneumoniae Conjunctivitis through the Lens of Community Ecology and Microbial Biogeography. Microorganisms. 2019 Dec 25; 8(1).
- Infectious corneal ulceration: a proposal for neglected tropical disease status. Bull World Health Organ. 2019 Dec 01; 97(12):854-856.
- Clinical metagenomics for infectious corneal ulcers: Rags to riches? Ocul Surf. 2020 01; 18(1):1-12.
- Authors' response: Povidone-Iodine for the Treatment of Microbial Keratitis. Surv Ophthalmol. 2019 Nov - Dec; 64(6):892-893.
- Real-Time Multiplex PCR Analysis in Infectious Uveitis. Semin Ophthalmol. 2019; 34(4):252-255.
- The persistent dilemma of microbial keratitis: Global burden, diagnosis, and antimicrobial resistance. Surv Ophthalmol. 2019 May - Jun; 64(3):255-271.
- Staphylococcus aureus from ocular and otolaryngology infections are frequently resistant to clinically important antibiotics and are associated with lineages of community and hospital origins. PLoS One. 2018; 13(12):e0208518.
- Resolution of fluoroquinolone-resistant Escherichia coli keratitis with a PROSE device for enhanced targeted antibiotic delivery. Am J Ophthalmol Case Rep. 2018 Dec; 12:73-75.
- Rapid Detection and Identification of Uveitis Pathogens by Qualitative Multiplex Real-Time PCR. Invest Ophthalmol Vis Sci. 2018 01 01; 59(1):582-589.
- Identification of the Infection Source of an Outbreak of Mycobacterium Chelonae Keratitis After Laser in Situ Keratomileusis. Cornea. 2018 Jan; 37(1):116-122.
- Methicillin-resistant Staphylococcus aureus in acute otitis externa. World J Otorhinolaryngol Head Neck Surg. 2018 Dec; 4(4):246-252.
- Neither non-toxigenic Staphylococcus aureus nor commensal S. epidermidi activates NLRP3 inflammasomes in human conjunctival goblet cells. BMJ Open Ophthalmol. 2017; 2(1):e000101.
- Detection of herpes simplex-1 and -2 and varicella zoster virus by quantitative real-time polymerase chain reaction in corneas from patients with bacterial keratitis. Arq Bras Oftalmol. 2017 Mar-Apr; 80(2):84-87.
- Analysis of Intraocular Lens Biofilms and Fluids After Long-Term Uncomplicated Cataract Surgery. Am J Ophthalmol. 2016 09; 169:46-57.
- Biofilms in infections of the eye. Pathogens. 2015 Mar 23; 4(1):111-36.
- Nosocomial bloodstream infections in a nationwide study: comparison between solid organ transplant patients and the general population. Transpl Infect Dis. 2015 Apr; 17(2):308-13.
- Unencapsulated Streptococcus pneumoniae from conjunctivitis encode variant traits and belong to a distinct phylogenetic cluster. Nat Commun. 2014 Nov 12; 5:5411.
- DNA extraction methods for panbacterial and panfungal PCR detection in intraocular fluids. Curr Eye Res. 2015 Jul; 40(7):697-706.
- Demonstration of plasmid-mediated drug resistance in Mycobacterium abscessus. J Clin Microbiol. 2014 May; 52(5):1727-9.
- Characterization of ocular methicillin-resistant Staphylococcus epidermidis isolates belonging predominantly to clonal complex 2 subcluster II. J Clin Microbiol. 2014 May; 52(5):1412-7.
- Prevalence and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus in ocular infections. Arq Bras Oftalmol. 2013 Nov-Dec; 76(6):350-3.
- Fusarium keratitis in Brazil: genotyping, in vitro susceptibilities, and clinical outcomes. Clin Ophthalmol. 2013; 7:1693-701.
- Nosocomial bloodstream infections in Brazilian pediatric patients: microbiology, epidemiology, and clinical features. PLoS One. 2013; 8(7):e68144.
- Emerging 8-methoxyfluoroquinolone resistance among methicillin-susceptible Staphylococcus epidermidis isolates recovered from patients with endophthalmitis. J Clin Microbiol. 2013 Sep; 51(9):2959-63.
- Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis. Clin Ophthalmol. 2012; 6:1907-14.
- Safety and efficacy of moxifloxacin-dexamethasone eyedrops as treatment for bacterial ocular infection associated with bacterial blepharitis. Adv Ther. 2012 May; 29(5):416-26.
- Cation concentration variability of four distinct Mueller-Hinton agar brands influences polymyxin B susceptibility results. J Clin Microbiol. 2012 Jul; 50(7):2414-8.
- Pseudomonas aeruginosa endophthalmitis after penetrating keratoplasty transmitted from the same donor to two recipients confirmed by pulsed-field gel electrophoresis. J Clin Microbiol. 2011 Sep; 49(9):3346-7.
- Real-time polymerase chain reaction test to discriminate between contamination and intraocular infection after cataract surgery. J Cataract Refract Surg. 2011 Jul; 37(7):1244-50.
- Nosocomial bloodstream infections in Brazilian hospitals: analysis of 2,563 cases from a prospective nationwide surveillance study. J Clin Microbiol. 2011 May; 49(5):1866-71.
- Detection and gram discrimination of bacterial pathogens from aqueous and vitreous humor using real-time PCR assays. Invest Ophthalmol Vis Sci. 2011 Feb 22; 52(2):873-81.
- Microbial profile and antibiotic susceptibility of culture-positive bacterial endophthalmitis. Eye (Lond). 2011 Mar; 25(3):382-7; quiz 388.
- Incidence of endophthalmitis after cataract surgery (2002-2008) at a Brazilian university-hospital. Arq Bras Oftalmol. 2010 Nov-Dec; 73(6):505-7.
- Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis. Arq Bras Oftalmol. 2009 Sep-Oct; 72(5):734-40.
- Advances in the microbiological diagnosis of sepsis. Shock. 2008 Oct; 30 Suppl 1:41-6.
- [Culture proven bacterial endophthalmitis: a 6-year review]. Arq Bras Oftalmol. 2008 Sep-Oct; 71(5):617-22.
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