Functional Genomics Core (Zebrafish Facility)

The Functional Genomics Core provides expert assistance using zebrafish as a model system to demonstrate functional effects of human disease-related gene variants and to model human inherited ocular disorders. The core provides zebrafish husbandry and assists investigators with project planning including morpholino design and design of guide RNAs for CRISPR/cas9 gene editing, training for zebrafish embryo injection and other manipulations and characterization of genetically modified fish including measurement of visual acuity and intraocular pressure.  

Services provided by the core:

  • Housing and general care of zebrafish (feeding, breeding, health monitoring, system maintenance)
  • Training in zebrafish handling and husbandry
  • Assistance with zebrafish protocol planning and preparation
  • Propagation of wild-type zebrafish stocks for embryo production
  • Importing and maintenance of transgenic or mutant lines
  • Fertilized wild type embryos for injection
  • Microinjection of zebrafish embryos
  • Morpholino-based gene knockdown strategies (including assistance with design of morpholinos for specific genes, evaluation of knockdown efficiency on mRNA level, and generation of constructs to obtain wild-type and mutant mRNA for the human orthologues for complementation assays)
  • CRISPR/Cas9 genome editing and establishment of edited lines (including assistance with design and generation of guide RNAs to target specific genes, and evaluation of CRISPR/Cas9 efficiency in vivo)
  • Molecular biology and histology (in situ hybridization, histological, or immunohistological analyses)
  • Vision assessment (visual motor response)
  • Intraocular pressure measurement

Contacts:

Kinga Bujakowska (Kinga_Bujakowska@meei.harvard.edu)
Anna Larson (Anna_Larson@meei.harvard.edu)