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Araldite Embedding

This preparation provides excellent morphological preservation for light microscopic examination of sectioned material, and sections can be re-embedded for ultrathin sectioning and electron microscopy. Fixed tissue is osmicated, decalcified, dehydrated, and infiltrated via a graded series of propylene oxide : araldite solutions, before being hardened in a 60 degree C oven. Cured blocks may be sectioned at thicknesses from ultrathin to 100 um.  Download Protocol (PDF).